高效液相色谱法测定冻干人凝血酶制品中甘氨酸含量
投稿时间:2019-03-12  修订日期:2019-05-11  点此下载全文
引用本文:
摘要点击次数: 171
全文下载次数: 0
作者单位E-mail
盛凤仙 上海新兴医药股份有限公司 wlrssfx@163.com 
基金项目:]上海市高新技术成果转化项目(编号20020461)
中文摘要:【】 目的 建立高效液相色谱法测定冻干人凝血酶制品中甘氨酸含量的方法。方法 采用Shim-Pack CLC-ODS色谱柱,以丙氨酸为内标,2、4二硝基氟苯(DNFB)为柱前衍生剂,50%乙腈溶液-0.05mol/L醋酸钠缓冲液(35︰65)为流动相,检测波长为360nm。结果 甘氨酸在0.006-0.030mg/ml浓度范围内线性关系良好(r=0.9996),平均回收率为100.4%,RSD为0.44%(n=9)。结论 该方法简单灵敏,结果准确可靠,可用于冻干人凝血酶制品中甘氨酸含量的测定。
中文关键词:冻干人凝血酶  甘氨酸  丙氨酸  高效液相色谱法  测定
 
Determination of glycine in human thrombin freeze-dried by HPLC SHENG Fengxian YANG Xiong ZHANG Youjie(Shanghai Xinxing Medicine Co.,Ltd, Shanghai 200135,China)
Abstract:【】 Objective To establish a method for determination the contents of glycine in the human thrombin freeze-dried by HPLC. Methods The analysis was carried on a Shim-Pack CLC-ODS column with a mobile phase of 50% acetonitrile -0.05mol/L sodium acetate buffer (35:65) at the detection wavelength of 360 nm, using alanine and 2,4-dinitrofluorobenzene as the internal standard and derivating agent, respectively. Results The standard curve was linear in the range of 0.006~0.030 mg/ml (r = 0.9996) for glycine. The average recovery was 100.4%, with RSD was 0.44% (n = 9). Conclusion This method was simple, sensitive, accurate, reliable, and suitable for determination of glycine in human thrombin freeze-dried.
keywords:human thrombin freeze-dried  glycine  alanine  HPLC  determination
  查看/发表评论  下载PDF阅读器
相关附件:   万方20190312(37.24%)  附件1稿件201903048初审意见修改内容的回复信  万方20190408_审稿(35.74%)  附件1稿件201903048复审意见修改内容的回复信  万方20190508_审稿(33.82%)  附件1稿件201903048重审意见修改内容的回复信  万方20190511_审稿(33.35%)
关闭

分享按钮