用HPLC法测定百蕊草中3个黄酮苷的含量
投稿时间:2019-01-23  修订日期:2019-03-06  点此下载全文
引用本文:宣伟东,成熙,叶曙娜,徐德东.用HPLC法测定百蕊草中3个黄酮苷的含量[J].药学实践杂志,2019,37(5):460~463
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作者单位
宣伟东 海军军医大学附属长海医院药学部, 上海 200081 
成熙 海军军医大学附属长海医院药学部, 上海 200081 
叶曙娜 海军军医大学附属长海医院药学部, 上海 200081 
徐德东 海军军医大学附属长海医院药学部, 上海 200081 
中文摘要:目的 建立HPLC法同时测定百蕊草中3个黄酮苷类化合物的含量。方法 色谱柱为DIKMA C18柱(250 mm×4.6 mm,5 μm),流动相为乙腈-水(21∶79,V/V),用冰醋酸调节pH至4.61,流速:1.0 ml/min,柱温:25℃,检测波长:346 nm。结果3个黄酮苷化合物山奈酚-3-O-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖苷(百蕊草素Ⅰ)、山奈酚-3-O-β-D-吡喃葡萄糖苷(紫云英苷)、山奈酚-3-O-L-吡喃鼠李糖基(1→2)-[6-O-乙酰基]-β-D-吡喃葡萄糖苷(6'-乙酰氧基百蕊草素Ⅰ)的进样浓度分别在125~200、2.5~40、5.0~80 μg/ml范围内线性关系良好(r=0.999 9),平均加样回收率分别为101.63%、99.82%和102.02%,RSD分别为1.72%、2.34%和1.94%(n=6)。结论 该方法准确,稳定性、重现性好,可作为百蕊草药材及制剂的质控方法。
中文关键词:百蕊草  黄酮苷  高效液相色谱  含量测定
 
Determination of three flavonoid glycosides in Thesium chinensis Turcz by HPLC method
Abstract:Objective To establish an assay method for three flavonoid glycosides:kaempferol-3-O-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside,kaempferol-3-O-glucoside and kaemperol-3-O-α-L-rhamnopyranosyl(1→2)-[6-O-acetyl]-β-D-glucopyranoside in Thesium chinensis Turcz by HPLC.Methods Dikma C18 column (520 mm×4.6 mm,5 μm) was used with the mobile phase of acetonitrile-water (21:79,adjusted pH to 4.61 with acetic acid) at a flow rate of 1.0 ml/min.The detection wavelength was set at 346 nm,and the column temperature was 25℃.Results The linear range of those three flavonoid glycosides was 125-200 μg/ml,2.5-40 μg/ml and 5.0-80 μg/ml (r=0.999 9).The average recoveries (n=6) were 101.63%,99.82% and 102.02%.RSD was 1.72%,2.34% and 1.94%(n=6) respectively.Conclusion This method is simple and accurate.It can be used for the quality control of Thesium chinensis Turcz and related preparations.
keywords:Thesium chinensis Turcz  flavonoid glycosides  HPLC  content determination
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