用HPLC法测定丹参素棕榈醇酯脂质体药物含量
投稿时间:2019-02-14  修订日期:2019-05-06  点此下载全文
引用本文:王晶,张川,栗意,任金妹,唐扣明.用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J].药学实践杂志,2019,37(5):450~452,480
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作者单位E-mail
王晶 复旦大学附属中山医院青浦分院药剂科, 上海 201700  
张川 上海大学医学院, 上海 200003  
栗意 上海健康医学院附属周浦医院, 上海 201318  
任金妹 复旦大学附属中山医院青浦分院药剂科, 上海 201700  
唐扣明 复旦大学附属中山医院青浦分院药剂科, 上海 201700 menshishan@126.com 
基金项目:上海市卫生和计划生育委员会科研项目资助(20164Y0101)
中文摘要:目的 建立HPLC法测定丹参素棕榈醇酯脂质体中药物含量的方法。方法 采用Agilent ZORBAX Eclipse Plus C18柱(4.6 mm×200 mm,5 μm);以乙腈-水(50∶50)含0.02%甲酸和0.02%三乙胺水、以pH 6~7的溶液为流动相,流速为1 ml/min,检测波长为280 nm,柱温为室温。结果 本色谱条件下丹参素棕榈醇酯与辅料及溶剂分离度符合要求,在0.05~0.45 mg/ml范围内线性关系良好(r=0.999 5,n=5),回收率在96%~102%之间,日内及日间RSD均小于2%(n=5)。3批样品的含量分别为97.81%、101.20%、98.53%。结论 该方法准确可靠、简单快速,可用于丹参素棕榈醇酯脂质体含量的测定。
中文关键词:丹参素  脂质体  高效液相色谱  包封率
 
Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method
Abstract:Objective To establish a HPLC method for the assay of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate (PDSS) in liposomes.Methods The separation was performed with an Agilent ZORBAX Eclipse Plus C18 column (4.6 mm×200 mm,5 μm) at room temperature.The mobile phase was acetonitrile-aqueous solution (500 ml water + 0.1 ml triethylamine + 0.1 ml formic acid,pH=6-7) (50:50) with the flow rate of 1 ml/min.Zidovudine palmitate was detected at 280 nm.Results PDSS had a good linear recovery between 0.05-0.45 mg/ml.The intra-day and inter-day RSD were <2%.The extracted recovery was in the range of 96% to 102%.The assay results for three batches were 97.81%,101.20% and 98.53%. Conclusion This method is simple,accurate and reliable.It can be used for the determination of PDSS in liposomes.
keywords:Danshensu  liposome  HPLC  entrapment efficiency
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