来曲唑联合45%高脂饲料诱导生殖内分泌及代谢表型异常的多囊卵巢综合征大鼠模型研究
投稿时间:2018-12-15  修订日期:2019-04-22  点此下载全文
引用本文:尹萍,路璐,陈媛媛,李丽,李赟,童国庆.来曲唑联合45%高脂饲料诱导生殖内分泌及代谢表型异常的多囊卵巢综合征大鼠模型研究[J].药学实践杂志,2019,37(5):422~426
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尹萍 上海中医药大学附属曙光医院, 上海 200120  
路璐 上海中医药大学附属曙光医院, 上海 200120  
陈媛媛 上海中医药大学附属曙光医院, 上海 200120  
李丽 上海中医药大学附属曙光医院, 上海 200120  
李赟 上海中医药大学附属曙光医院, 上海 200120  
童国庆 上海中医药大学附属曙光医院, 上海 200120 drivftongguoqing@hotmail.com 
基金项目:上海中医药大学2016年预算内项目(2016N47)
中文摘要:目的 建立来曲唑联合高脂饲料诱导SD雌性大鼠多囊卵巢综合征(PCOS)内分泌及代谢异常的动物模型。方法 选取18只21日龄雌性大鼠喂养45%高脂饲料16周,第6周龄开始服用来曲唑直至造模结束;8只对照组大鼠常规饲料喂养,第6周龄服用生理盐水。观察阴道涂片及卵巢组织学变化,比较每周体重变化,测定脏器重量、血清睾酮水平、糖耐量、血脂水平、空腹胰岛素以及胰岛素敏感指数。结果 与对照组比较,模型组大鼠体重增加,卵巢、腹股沟脂肪质量增加,子宫、垂体质量及脏器指数显著减少。模型组大鼠卵巢呈多囊样改变,颗粒细胞层减少,白膜增加。模型组大鼠的三酰甘油和低密度脂蛋白升高,糖耐量曲线下面积增加,血清睾酮水平上升,空腹胰岛素水平升高,胰岛素敏感性降低。结论 来曲唑联合高脂饲料可以建立有效的内分泌及代谢表型异常PCOS动物模型。
中文关键词:来曲唑  高脂饲料  多囊卵巢综合征  内分泌  糖脂代谢  动物模型
 
Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder
Abstract:Objective To establish rat polycystic ovary syndrome model with letrozole combined high fat diet.Methods Eighteen 21-day-old female rats were fed with 45% high-fat diet for 16 weeks.Letrozole was started at the 6th week of age until the end of modeling.Eight rats in control group were fed with regular diet and Saline without letrozole at the 6th week of age.The vaginal smear and ovarian histological changes were investigated.The changes in body mass,organ weight,blood testosterone level,glucose tolerance,blood lipid level,fasting insulin and insulin sensitivity index were measured.Results In the model group,the ovary of the rats showed a multi-cystic change,the granulosa cell layer decreased,and the tunica albuginea increased.Compared with the control group,the body weight of the model group increased,the weight of the ovary and inguinal fat increased,the weight and weight index of the uterus and pituitary gland decreased,the triglyceride and low density lipoprotein increased,the area under the glucose tolerance curve increased,the blood testosterone level increased,the fasting insulin increased,and the insulin sensitivity decreased.Conclusion Letrozole combined with high fat diet could establish an effective PCOS model of abnormal endocrine and metabolic phenotype.
keywords:letrozole  high-fat diets  polycystic ovary syndrome  reproductive endocrine  glucose and lipid metabolism  animal model
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